anti myc agarose (Thermo Fisher)
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anti myc agarose
Anti Myc Agarose, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+myc+agarose/Agarose/pmc13131402-117-3-8
Average 99 stars, based on 1 article reviews
Anti Myc Agarose, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+myc+agarose/Agarose/pmc13131402-117-3-8
Average 99 stars, based on 1 article reviews
anti myc agarose - by Bioz Stars,
2026-11
99/100 stars
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Incubation:Article Title: The Src1-PGC1α-AP1 complex-dependent secretion of substance P induces inflammation and apoptosis in encephalomyocarditis virus-infected mice. Article Snippet: Substance P (SP), a neuropeptide consisting of 11 amino acid residues, is involved in the pathogenesis of encephalomyocarditis virus (EMCV)-induced myocarditis by stimulating the production of proinflammatory cytokines.. However, the underlying mechanism that regulates SP production is still unknown.. In this study, we report the transcriptional regulation of the Tachykinin Precursor 1 (TAC1) gene that encodes SP by a transcriptional complex composed of Steroid Receptor Coactivator 1 (Src1), Peroxisome proliferator-activated receptor-gamma coactivator 1 (PGC1α), and Activator Protein 1 (AP1) transcription factor. Immunoprecipitation:Article Title: The Src1-PGC1α-AP1 complex-dependent secretion of substance P induces inflammation and apoptosis in encephalomyocarditis virus-infected mice. Article Snippet: Substance P (SP), a neuropeptide consisting of 11 amino acid residues, is involved in the pathogenesis of encephalomyocarditis virus (EMCV)-induced myocarditis by stimulating the production of proinflammatory cytokines.. However, the underlying mechanism that regulates SP production is still unknown.. In this study, we report the transcriptional regulation of the Tachykinin Precursor 1 (TAC1) gene that encodes SP by a transcriptional complex composed of Steroid Receptor Coactivator 1 (Src1), Peroxisome proliferator-activated receptor-gamma coactivator 1 (PGC1α), and Activator Protein 1 (AP1) transcription factor. Article Title: USP24-dependent stabilization of Runx2 recruits a p300/NCOA3 complex to transactivate ADAMTS genes and promote degeneration of intervertebral disc in chronic inflammation mice Article Snippet: .. The cell lysates were immunoprecipitated by exposure to anti-Flag agarose (Sigma-Aldrich; #A4596) and other:Article Title: Epigenetically silenced KAT2B suppresses de novo lipogenesis through destroying HDAC5/LSD1 complex assembly in renal cell carcinoma. Article Snippet: Then, the dried peptide samples were used for highperformance liquid chromatography using Article Title: Epigenetically silenced KAT2B suppresses de novo lipogenesis through destroying HDAC5/LSD1 complex assembly in renal cell carcinoma Article Snippet: Cells were treated with 100 μM CHX (HY-12320, MCE, USA) for 0, 1, 2, 3, and 4 h respectively, and collected for protein extraction and western blots analysis. Purification:Article Title: MAVS deSUMOylation by SENP1 inhibits its aggregation and antagonizes IRF3 activation. Article Snippet: Mitochondrial antiviral signaling protein (MAVS) is an adapter that recruits and activates IRF3.. However, the mechanisms underpinning the interplay between MAVS and IRF3 are largely unknown.. Here we show that small ubiquitin-like modifier (SUMO)-specific protease 1 negatively regulates antiviral immunity by deSUMOylating MAVS. Protease Inhibitor:Article Title: Coronavirus envelope protein activates TMED10-mediated unconventional secretion of inflammatory factors Article Snippet: .. The reagents used in this study were purchased from the following sources: DSS (Thermo, 21655), GTP (Roche, 11140957001), ATP (Sigma, A2383), Creatine phosphate (Calbiochem, 2380), Creatine Kinase (Roche, 10127566001), Proteinase K (ABCone, P78893), Phenylmethylsulfonyl fluoride (PMSF) (Beyotime, ST505), Opti-Prep (Serumwerk Bernburg AG, 1893), Protease inhibitor cocktail (Roche, 11697498001), Phosphotase inhibitors (Roche, 4906845001), LPS (Sigma, L2880), Mouse IL-1 beta Uncoated ELISA Kit (Thermo, 88-7013-22), anti-V5 agarose (Sigma, A7345), Article Title: Coronavirus envelope protein activates TMED10-mediated unconventional secretion of inflammatory factors. Article Snippet: .. The reagents used in this study were purchased from the following sources: DSS (Thermo, 21655), GTP (Roche, 11140957001), ATP (Sigma, A2383), Creatine phosphate (Calbiochem, 2380), Creatine Kinase (Roche, 10127566001), Proteinase K (ABCone, P78893), Phenylmethylsulfonyl fluoride (PMSF) (Beyotime, ST505), Opti-Prep (Serumwerk Bernburg AG, 1893), Protease inhibitor cocktail (Roche, 11697498001), Phosphotase inhibitors (Roche, 4906845001), LPS (Sigma, L2880),Mouse IL-1 beta Uncoated ELISA Kit (Thermo, 88-7013- 22), anti-V5 agarose (Sigma, A7345), Enzyme-linked Immunosorbent Assay:Article Title: Coronavirus envelope protein activates TMED10-mediated unconventional secretion of inflammatory factors Article Snippet: .. The reagents used in this study were purchased from the following sources: DSS (Thermo, 21655), GTP (Roche, 11140957001), ATP (Sigma, A2383), Creatine phosphate (Calbiochem, 2380), Creatine Kinase (Roche, 10127566001), Proteinase K (ABCone, P78893), Phenylmethylsulfonyl fluoride (PMSF) (Beyotime, ST505), Opti-Prep (Serumwerk Bernburg AG, 1893), Protease inhibitor cocktail (Roche, 11697498001), Phosphotase inhibitors (Roche, 4906845001), LPS (Sigma, L2880), Mouse IL-1 beta Uncoated ELISA Kit (Thermo, 88-7013-22), anti-V5 agarose (Sigma, A7345), Article Title: Coronavirus envelope protein activates TMED10-mediated unconventional secretion of inflammatory factors. Article Snippet: .. The reagents used in this study were purchased from the following sources: DSS (Thermo, 21655), GTP (Roche, 11140957001), ATP (Sigma, A2383), Creatine phosphate (Calbiochem, 2380), Creatine Kinase (Roche, 10127566001), Proteinase K (ABCone, P78893), Phenylmethylsulfonyl fluoride (PMSF) (Beyotime, ST505), Opti-Prep (Serumwerk Bernburg AG, 1893), Protease inhibitor cocktail (Roche, 11697498001), Phosphotase inhibitors (Roche, 4906845001), LPS (Sigma, L2880),Mouse IL-1 beta Uncoated ELISA Kit (Thermo, 88-7013- 22), anti-V5 agarose (Sigma, A7345), |
![Ded1p is not detectably acetylated in vitro. a and b) Ded1p tagged with FLAG (pAKH320) was immunoprecipitated from BY4741 yeast either expressing endogenous (YEP24) or constitutively overexpressed levels of HAT1 (pAKH257). The samples were assessed for the presence of acetylated proteins using an antiacetyl lysine pan antibody. The levels of immunoprecipitated Ded1p were visualized by an anti-Ded1p antibody. Purified acetylated BSA (50 ng) and recombinant Ded1p (800 ng) were used as controls for the antibodies. Yeast cells were grown to mid-log phase in selective media without stress unless indicated. Stresses included 30 min of high heat stress at 46 °C (hs) or DTT for 30 min or 3 h. c) Yeast lysate containing no HAT1 (yAKH201 with YEP24) or containing myc-tagged Hat1p (yAKH201 + pAKH834) was <t>incubated</t> <t>with</t> <t>anti-c-Myc</t> agarose resin to immobilize Hat1p, if present. Resin was incubated with 2.5 mM [ 3 H] acetyl coenzyme A and 0.2 mg/ml of either human histone H4 or recombinant Ded1p for 60 min at 37 °C. Protein was captured on chromatography paper, and tritium levels were quantified by liquid scintillation counting. Bars represent the average of triplicates; error bars represent the SD. P -values less than 0.05 as calculated by an unpaired two-tailed t -test are marked with an asterisk.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8820/pmc12958820/pmc12958820__jkaf307f2.jpg)